Equipment:
- Fermentors 500ml to 1L working volume, 10x--Our own
- Fermentor 15L working volume--HKPolyU
- 磁力搅拌加热锅 5x big enough for 2L Duran bottle
- New centrifuge bottles--500ml 20x
- New 2L Duran bottles for fermentation GL80 2000ml 10x
- Stainless steel spargers 20x
- silicon tubings
- air filters 100x
- Red caps 50x
- Air pumps
- Air distributors
- Microscope
TaoBao links to GL80 Bottles:https://detail.tmall.com/item.htm?_u=a1kh5dkiba65&id=613305790505&spm=a1z09.2.0.0.20bf2e8dEObjon&skuId=4317741789753
Materials:
- Molasses
- Peptone?
- Antifoam A
- Papain
- Chemicals and reagents as listed in the tables below
Yeast culture medium:
Optimized Medium:
Mineral mixture:
