Phase 1--December 2023 to May 2024
1A. December 2023 to 12 January 2024
- Isolate and identify the yeast strain that is nearly identical to that of the Reference Yeast Extract
- Streak from source for single colonies
- YPD plates
- Pick single colony
- Observe under microscope
- Grow test-tube cultures in YPD medium aerobically
- Extract DNA from yeast cells
- Extract DNA from Reference Yeast Extract
- Interdelta Fingerprint comparison
- Observe the patterns of lower bands
- Nanopore sequencing for WGS
- DNA sequence comparison-- target >90% similarity
1B. 15 January 2024 to 26 January 2024
- Lab-scale fermentor cultivation
- Medium follows the previous optimized recipe
- 4x500ml fermentors for as many times as needed for >20g of Yeast extract--Need about 10L of culture--5 times
- Collect yeast cells by centrifugation
- Pool enough yeast cells
- Autolysis of yeast cells to make yeast extracts, following the previous optimized treatment conditions
- Freeze-dry/oven-dry the yeast extract--check with Eliz
1C. 29 Janurary 2024 to 16 February 2024
- Large lab-scale fermenter cultivation
- HKPolyU fermenter familiarization
- HKPolyU fermenter test-runs
- >50g Yeast extracts production
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